Julie Sadino

Education

2016 – 2022

University of Colorado, Boulder
Boulder, Colorado
Focus: Bioinformatics, behavioral neuroscience, and molecular biology
Thesis advisor: Dr. Zoe Donaldson, Ph.D.

2010 – 2014

Linfield University
McMinnville, Oregon
B.S. Biochemistry and Molecular Biology
Minor, Studio Art
Focus: Genetics and material chemistry
Thesis advisor: Dr. Anne Kruchten, Ph.D.

2013

Canterbury University
Christchurch, New Zealand
Exchange semester

Skills

Computational

  • R
  • Command line
  • Linux
  • Python
  • ImageJ/FIJI
  • BASH scripting
  • Cluster computing
  • GitHub
  • end-to-end RNA sequencing analysis
  • Statistical modeling
  • Automated animal tracking
  • Cell counting using ML software
  • Adobe Illustrator
  • Microsoft suite
  • Benchling

Animal

  • Sterotaxic neurosurgery for viral (AAV) injections
  • Tubal ligations
  • Tissue handling
  • Brain slicing
  • Perfusion
  • Animal handling and husbandry
  • Genotyping
  • Behavioral tests
    • Partner Preference Test
    • Resident intruder
    • Elevated plus maze
    • Forced swim test
    • Open field test

Molecular

  • Molecular cloning
  • Cell culture
  • KO stable cell line generation
  • CRISPR gRNA generation
  • Next generation sequencing (NGS)
  • Protein purification
  • Transfection
  • Immunoflouresence and histochemistry
  • RNA extraction from animal tissue and cell culture
  • cDNA library preparation
  • RT-qPCR
  • Western Blotting
  • High-throughput microscopy
  • Antibody optimization
  • Microscopy

Professional

  • Project management
  • People management
  • Grantsmanship
  • Written and oral communication
  • Collaboration
  • Equipment testing and purchasing
  • Standardized Operating Procedure (SOP) generation
  • Organization
  • Shared resource management
  • Version control
  • Notion
  • Customer service
  • Event planning